Microbial Contamination of Toothbrushes Used After Dental Extraction: a Comparative Study of Decontamination Methods

Abstract

Background: Toothbrushes used after dental extractions are prone to microbial contamination, which can compromise wound healing. This study investigates and compares the efficacy of three decontamination methods in reducing microbial load on toothbrushes post-extraction.

Methods: A total of 75 patients undergoing dental extraction were randomly assigned to three groups (n=25 each). Group A used toothbrushes without any decontamination (control), Group B rinsed brushes daily with 0.12% chlorhexidine, and Group C immersed brushes in 60°C hot water for 5 minutes daily. After 7 days, toothbrushes were cultured, and colony-forming units (CFU/mL) were measured. Statistical analysis was conducted using ANOVA and Tukey's post-hoc test.

Results: Group A (control) showed the highest mean CFU count (1,500,000 ± 300,000 CFU/mL), followed by Group D (600,000 ± 120,000 CFU/mL), and Group B (250,000 ± 80,000 CFU/mL). ANOVA showed significant differences among groups (p < 0.001). Tukey’s test confirmed chlorhexidine was significantly more effective than hot water and control.

Conclusion: Daily rinsing of toothbrushes with 0.12% chlorhexidine significantly reduces microbial contamination post-extraction and is more effective than hot water immersion. Toothbrush disinfection should be recommended as part of post-operative oral hygiene.